Computer Vision News - February 2023

44 ISBI 2023 Challenge almost exact registration of the image pairs for this volumetric analysis. “ Biologists traditionally only care about preparing a sample, imaging it once and that’s it, but nowwe reallywant togetmore information from these samples, ” Ruihan explains. “ We want to align two rounds of data together. This combinatorial encoding will be the future trend of biology. That’s “ When you download the image data, look at it in 3D to have a clearer idea of what the offset and deformation actually look like , ” she advises. “ It can be easier with volumes of this size to plot things in 2D in a Jupyter Notebook or something, but taking the time to overlay the volumes in a 3D space will help you to understand the deformation and how to approach registering it. The zebrafish data has mostly linear deformations, large rotations, and a large amount of offset in the Z-axis. The mouse data has the most nonlinear deformations, which is probably the most challenging. It also has the biggest difference in staining intensity and image quality. ” The Boyden lab routinely generates terabytes of data, and its papers are often cited, so it urgently needs registration to work perfectly. Registration is used for critical downstream tasks, such as creating a 3D reconstruction of the entire brain , so it is imperative to have a precise and